M30 CytoDeath™ ELISA

Apoptosis detection in cell cultures

The M30 CytoDeath™ ELISA is a novel product developed for cell culture applications. The assay has a dynamic range and sensitivity suitable for in vitro work.

The M30 CytoDeath™ ELISA is a powerful drug screening tool.  It is useful for in vitro characterization of apoptosis-inducing drugs, including establishment of time course kinetics and dose-response relationships. The effects of large numbers of siRNAs on apoptosis can be tested in the 96 well format. The assay offers a unique possibility to quantify apoptosis of epithelially derived cells in multicellular spheroids and organ culture systems (see Herrmann et al., J Biomolecular Screening 13, 1, 2008).

The M30 CytoDeath™ ELISA is based on the M30 antibody (detecting the Asp396 caspase-cleavage site on CK18) and antibody M6. This combination is different from that used in the M30-Apoptosense® ELISA.

The M30 CytoDeath™ ELISA is not suitable for serum/plasma.

Apoptosis Quantification in Multicellular Spheroids

Multicellular tumor spheroids represent models for the three-dimensional microenvironment of tumors. Spheroids are superior to monolayer cultures for applications such as anticancer drug research.

PEVIVA has in close collaboration with the Karolinska Institute developed a pioneering method for drug screening in multicellular spheroids. This combination of reproducible culture of multicellular spheroids and a robust quantification method of apoptotic cells with the M30 CytoDeath™ ELISA now broadens the researcher's horizons and allows studies on drug penetration and induction of cell death.

Further information can be found in our Spheroid Brochure