Products
PEVIVA is specialised in production and development of high end in vitro and in vivo biomarkers and antibodies.
M30 Apoptosense® ELISA(Prod. No. 10010) |
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M30 Apoptosense® ELISA is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of caspase-cleaved keratin 18 (ccK18, caspase-cleaved Cytokeratin 18, ccCK18, CK18Asp396-NE or M30 neo-epitope) levels in cytosolic cell extracts, cell culture supernatants, human serum or plasma or other body fluids. The M30 Apoptosense® ELISA uses the M30 antibody which is specific to this K18 cleavage site and does not react with viable or necrotic cells (Leers et al., 1999).
The M30 Apoptosense® ELISA measures the accumulation of a caspase-generated product resulting in an unmatched sensitivity thus allowing the tracing of apoptotic cells of epithelial origin in patient plasma/serum. The most important applications are cancer (Kramer et al., 2004; Kramer et al., 2006) and liver disease (Bantel et al. 2004; Wieckowska et al. 2006).
The assay is ideally suited for high through-put screening for anticancer drugs (Hägg et al. 2002; Erdal et al. 2005).
PEVIVA's M30 Apoptosense® ELISA assay is CE accredited as a medical device for in vitro diagnostic use.
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M65 EpiDeath® ELISA(Prod. No. 10040) |
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The M65 EpiDeath® ELISA is the next generation of Peviva's biomarkers. It is intended for the quantitative detection of keratin 18 (K18) present in cell culture supernatants, human serum, human plasma or other body fluids after it has been released from dying cells into the extracellular compartment. The assays offers the opportunity to quantify total cell death (due to apoptosis and necrosis) of K18 positive (epithelial) cells.
The M65 EpiDeath® ELISA, when used in combination with the M30 Apoptosense® ELISA (which specifically measures apoptosis), allows to quantify and distinguish different forms of epithelial cell death as both assay are standardised against the identical reference (Kramer et al., 2004).
The M65 EpiDeath® ELISA is a modified version of the M65® ELISA and is intended for the same applications. Further information about the similarities and differences between these two assaysis available on the website Comparison between M65 EpiDeath® ELISA and M65® ELISA.
The M65 EpiDeath® ELISA is registered as a medical device for in vitro diagnostic use in accordance with the CE-IVD directive.
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M65® ELISA(Prod. No. 10020) |
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The M65® ELISA is intended for the quantitative detection of keratin 18 (K18) present in cell culture supernatants, human serum, human plasma or other body fluids after it has been released from dying cells into the extracellular compartment. The M65® ELISA offers the opportunity to quantify total cell death (due to apoptosis and necrosis) of K18 positive (epithelial) cells.
The M65® ELISA when used in combination with the M30 Apoptosense® ELISA (which specifically measures apoptosis) allows to quantify and distinguish different forms of epithelial cell death as both assays are standardized against the identical reference (Kramer et al., 2004).
PEVIVA's M65® ELISA assay is CE accredited as a medical device for in vitro diagnostic use.
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M30 CytoDeath™ ELISA(Prod. No. 10900) |
The M30 CytoDeath™ ELISA is a novel product developed for cell culture applications. The assay has a dynamic range and sensitivity suitable for in vitro work.
The M30 CytoDeath™ ELISA is a powerful drug screening tool and is useful for in vitro characterisation of apoptosis-inducing drugs. The assay offers a unique possibility to quantify apoptosis of epithelially derived cells in multicellular spheroids and organ culture systems.
The M30 CytoDeath™ ELISA is based on the M30 antibody (detecting the Asp396 caspase-clevage site on K18) and antibody M6.
The M30 CytoDeath™ ELISA is not suitable for serum/plasma.
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M30 CytoDEATH™ antibody(Prod. No. 10700/10750/10800/10830) |
The M30 CytoDEATH™ monoclonal antibody recognizes a neo-epitope formed after caspase cleavage of K18 in apoptotic cells (K18Asp396-NE: M30 neo-epitope). M30 CytoDEATH™ does not detect native, unmodified K 18 present in viable or necrotic cells (Leers et al., 1999). Consequently, M30 CytoDEATH™ is a versatile tool for simple and reliable determination of apoptosis of cells of epithelial origin, both for cultured cells and for formalin fixed tissue sections.
The M30 CytoDEATH™ products are provided in a convenient ready-to-use stock solution for 200 tests .
» M30 CytoDEATH™ (unlabelled), prod. no. 10700
» M30 CytoDEATH™ Biotin, prod. no. 10750
» M30 CytoDEATH™ Fluorescein, prod. no. 10800
» M30 CytoDEATH™ Orange, prod. no. 10830
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M5/M6 Keratin 18 antibodies(Prod. No. 10600/10650) |
Keratins build up the cytoskeleton of epithelial cells. Keratin 18 (K18), a type I keratin, is a common part of the intermediate filament, often in combination with keratin 8. K18 is used to identify cells of epithelial origin.
The M5 and M6 Keratin 18 mouse monoclonal antibodies bind to conventional epitopes on Keratin 18 between the caspase-cleavage sites aa238 and aa396. The antibodies are used for staining of epithelial cells by immunohistochemistry and for western blotting. The M5 Keratin 18 antibody is specific for the human and monkey protein, whereas the M6 Keratin 18 antibody also binds to mouse, rat and dog K18.
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