Products
PEVIVA is specialised in production and development of high end in vitro and in vivo biomarkers and antibodies.
M30-Apoptosense® ELISA |
(Prod. No. 10010) |
M30-Apoptosense® is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of caspase-cleaved cytokeratin-18 (CK18Asp396-NE: M30 neo-epitope) levels in cytosolic cell extracts, cell culture supernatants, human serum or plasma or other body fluids. The M30-Apoptosense® ELISA uses the M30 antibody which is specific to this CK18 cleavage site and does not react with viable or necrotic cells (Leers et al., 1999).
The M30-Apoptosense® ELISA measures the accumulation of a caspase-generated product resulting in an unmatched sensitivity thus allowing the tracing of apoptotic cells of epithelial origin in patient plasma/serum. The most important applications are cancer (Kramer et al., 2004; Kramer et al., 2006) and liver disease (Bantel et al. 2004; Wieckowska et al. 2006).
The assay is ideally suited for high through-put screening for anticancer drugs (Hägg et al. 2002; Erdal et al. 2005).
PEVIVA's M30-Apoptosense® ELISA assay is CE accredited as a medical device for in vitro diagnostic use.
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M65® ELISA |
(Prod. No. 10020) |
The M65® ELISA is intended for the quantitative detection of cytokeratin 18 (CK18) present in cell culture supernatants, human serum, human plasma or other body fluids after it has been released from dying cells into the extracellular compartment. The M65® ELISA offers the opportunity to quantify total cell death (due to apoptosis and necrosis) of CK18 positive (epithelial) cells.
The M65® ELISA when used in combination with the M30-Apoptosense® assay (which specifically measures apoptosis) allows to quantify and distinguish different forms of epithelial cell death as both assays are standardized against the identical reference (Kramer et al., 2004).
PEVIVA's M65® ELISA assay is CE accredited as a medical device for in vitro diagnostic use.
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M30 CytoDeath™ ELISA |
(Prod. No. 10900) |
The M30 CytoDeath™ ELISA is a novel product developed for cell culture applications. The assay has a dynamic range and sensitivity suitable for in vitro work.
The M30 CytoDeath™ ELISA is a powerful drug screening tool and is useful for in vitro characterization of apoptosis-inducing drugs. The assay offers a unique possibility to quantify apoptosis of epithelially derived cells in multicellular spheroids and organ culture systems.
The M30 CytoDeath™ ELISA is based on the M30 antibody (detecting the Asp396 caspase-clevage site on CK18) and antibody M6.
The M30 CytoDeath™ ELISA is not suitable for serum/plasma.
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M30 CytoDEATH™ antibody |
(Prod. No. 10700/10750/ 10800/10830/10850) |
The M30 CytoDEATH™ monoclonal antibody recognizes a neo-epitope formed after caspase cleavage of CK18 in apoptotic cells (CK18Asp396-NE: M30 neo-epitope). M30 CytoDEATH™ does not detect native, unmodified CK 18 present in viable or necrotic cells (Leers et al., 1999). Consequently, M30 CytoDEATH™ is a versatile tool for simple and reliable determination of apoptosis of cells of epithelial origin, both for cultured cells and for formalin fixed tissue sections.
The M30 CytoDEATH™ products are provided in a convenient ready-to-use stock solution for 200 tests .
» M30 CytoDEATH™ (unlabelled), prod. no. 10700
» M30 CytoDEATH™ Biotin, prod. no. 10750
» M30 CytoDEATH™ Fluorescein, prod. no. 10800
» M30 CytoDEATH™ Orange, prod. no. 10830
» M30 CytoDEATH™ Red, prod. no. 10850
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M5/M6 CytoKERATIN 18 antibodies |
(Prod. No. 10600 / 10650) |
Background
In eukaryotic cells, the cytoskeleton is composed of three different types of morphologically distinct filamentous structures: microfilaments, intermediate filaments (IF), and microtubules.
The epithelial cytokeratins (IF types I and II) are conserved phylogenetically and are closely related, biochemically and imunologically.
Cytokeratins 1 - 8 constitute the type II group (53 - 68 kDa, neutral to basic protein components), while cytokeratins 9 - 20 constitute the type I group (40 - 56 kDa, acidic proteins).
Upon release from dying cells, cytokeratins provide useful markers for epithelial malignancies, distinctly reflecting ongoing cellular turnover. Certain cytokeratins, the most prominent example being Cytokeratin 18, are substrates for lethal caspase activation and the subsequent release of defined protein fragments occurs during apoptotic cell death.
The clinical value of determining different soluble cytokeratin protein fragments in blood samples (i.e. using the M30-Apoptosense® ELISA or M65® ELISA) lies in the rapid and non invasive assessment of drug efficacy or response to therapy in epithelial cell carcinomas.
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